BARD1 Polyklonaler Antikörper

BARD1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 22964-1-AP

Synonyme

BARD 1, BARD-1, BRCA1 associated RING domain 1, BRCA1-associated RING domain protein 1, EC:2.3.2.27

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  WB analysis of SH-SY5Y using 22964-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22964-1-AP (BARD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

22964-1-AP bindet in WB, ELISA BARD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BARD1 fusion protein Ag18965
• Vollständiger Name: BRCA1 associated RING domain 1
• Berechnetes Molekulargewicht: 777 aa, 87 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC126426
• Gene symbol: BARD1
• Gene ID (NCBI): 580
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BARD1 (BRCA1-associated RING domain protein 1) is a multifunctional protein that interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. Moreover, BARD1 is central to DNA repair, tumor suppression, and cancer predisposition.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu
human	WB	Molecules; FOXO3a Mediates Homologous Recombination Repair (HRR) via Transcriptional Activation of MRE11, BRCA1, BRIP1, and RAD50; Authors - Gozde Inci
human	WB	Am J Physiol Cell Physiol; Synergistic Effects of Olaparib and Palbociclib in Resistant epithelial ovarian cancer; Authors - Shuo Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vikas (Verified Customer) (04-08-2025)

worked very well. Highly recommended.

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: HEK-293