BARHL2 Polyklonaler Antikörper

BARHL2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23976-1-AP

Synonyme

BarH like 2 homeobox protein, BarH like homeobox 2, BARHL2

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  WB analysis of HepG2 using 23976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23976-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23976-1-AP (BARHL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23976-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 23976-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23976-1-AP (BARHL2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 23976-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (23976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

23976-1-AP bindet in WB, IHC, IF/ICC, ELISA BARHL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BARHL2 fusion protein Ag21156
• Vollständiger Name: BarH-like homeobox 2
• Berechnetes Molekulargewicht: 387 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC126439
• Gene symbol: BARHL2
• Gene ID (NCBI): 343472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Aging; Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging; Authors - Meiyang Li