BARHL2 Rekombinanter Antikörper

BARHL2 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240558G8

Kat-Nr. : 83596-4-PBS

Synonyme

240558G8, BarH like 2 homeobox protein, BarH-like 2 homeobox protein

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  WB analysis using 83596-4-RR (same clone as 83596-4-PBS)

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83596-4-RR (BARHL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83596-4-RR (same clone as 83596-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BARHL2 antibody (83596-4-RR, Clone: 240558G8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83596-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00582-2

  Cytometric bead array standard curve of MP00582-2, BARHL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83596-1-PBS. Detection antibody: 83596-4-PBS. Standard: Ag21156. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83596-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83596-4-RR against Human BARHL2 were performed. The affinity constant is 0.478 nM.

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Geprüfte Anwendungen

Produktinformation

83596-4-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA BARHL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BARHL2 fusion protein Ag21156
• Vollständiger Name: BarH-like homeobox 2
• Berechnetes Molekulargewicht: 387 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC126439
• Gene symbol: BARHL2
• Gene ID (NCBI): 343472
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BARHL2 also named as BarH like homeobox 2 is 279 amino acid protein, which contains 1 homeobox DNA-binding domain. BARHL2 localizes in nucleus and belongs to BAR homeobox family. BARHL2 as a transcription factor binds optimally to the DNA consensus sequence. Barx2 may differentially control the expression of L1 and other target genes during embryonic development. Barx2 is expressed in several smooth muscle-containing tissues, as well as skeletal muscle, brain, tongue and esophagus.