DDX39B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DDX39B Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 14798-1-AP

Synonyme

ATP dependent RNA helicase p47, BAT1, D6S81E, DDX39, DDX39B, DEAD box protein UAP56, HLA B associated transcript 1, Spliceosome RNA helicase BAT1, UAP56

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14798-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14798-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14798-1-AP (UAP56 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14798-1-AP

  IP Result of anti-UAP56 (IP:14798-1-AP, 4ug; Detection:14798-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14798-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14798-1-AP (UAP56 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

14798-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA DDX39B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DDX39B fusion protein Ag6512
• Vollständiger Name: HLA-B associated transcript 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000361
• Gene symbol: BAT1
• Gene ID (NCBI): 7919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX39B, is a 428 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain and belongs to the DEAD box helicase family. DECD subfamily. DDX39B localizes in the nucleus and cytoplasm. BAT1 is involved in nuclear export of spliced and unspliced mRNA. BAT1 as ATP dependent RNA helicase, may be a translation initiation factor and acts as a negative regulator of inflammatory cytokines. This antibody may cross-react with DDX39A.

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	IF,ChIP	Genes Dev; UAP56/DDX39B is a major cotranscriptional RNA-DNA helicase that unwinds harmful R loops genome-wide.; Authors - Carmen Pérez-Calero
human,mouse	WB,IHC	Cell Death Discov; DDX39B contributes to the proliferation of colorectal cancer through direct binding to CDK6/CCND1.; Authors - Haonan Zhang ; KD Validated
human	WB	iScience; THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate; Authors - Mareike Polenkowski
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang
human	WB,IF,IP	Sci Rep; ALS Associated Mutations in Matrin 3 Alter Protein-Protein Interactions and Impede mRNA Nuclear Export.; Authors - Ashley Boehringer