BAX Monoklonaler Antikörper

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• KD/KO Validated

BAX Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G5E8

Kat-Nr. : 60267-1-PBS

Synonyme

4G5E8, Apoptosis regulator BAX, Bcl2-L-4, Bcl-2-like protein 4

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  WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  WB analysis using 60267-1-Ig (same clone as 60267-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60267-1-Ig (same clone as 60267-1-PBS)

  WB result of BAX antibody (60267-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAX transfected HeLa cells. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60267-1-Ig (same clone as 60267-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60267-1-Ig (same clone as 60267-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60267-1-Ig (BAX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 60267-1-Ig (same clone as 60267-1-PBS)

  IP result of anti-BAX (IP:60267-1-Ig, 4ug; Detection:60267-1-Ig 1:6000) with THP-1 cells lysate 1520 ug. This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 60267-1-Ig (BAX antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60267-1-Ig (same clone as 60267-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAX antibody (60267-1-Ig, Clone: 4G5E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 60267-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60267-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA BAX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BAX fusion protein Ag21068
• Vollständiger Name: BCL2-associated X protein
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC014175
• Gene symbol: BAX
• Gene ID (NCBI): 581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).