BAZ2A Rekombinanter Antikörper

BAZ2A Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230205H8

Kat-Nr. : 82910-1-RR

Synonyme

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

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  IF Staining of HepG2 using 82910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

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  IF Staining of A549 using 82910-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

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  Affinity and Kinetic Characterization of 82910-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82910-1-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82910-1-RR bindet in IF/ICC, ELISA BAZ2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BAZ2A fusion protein Ag33963
• Vollständiger Name: bromodomain adjacent to zinc finger domain, 2A
• Berechnetes Molekulargewicht: 211 kDa
• GenBank-Zugangsnummer: NM_013449.4
• Gene symbol: BAZ2A
• Gene ID (NCBI): 11176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.