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BBOF1 Polyklonaler Antikörper

BBOF1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24337-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of TT cells using BBOF1 Polyclonal antibody (24337-1-AP)

    WB analysis of TT using 24337-1-AP (same clone as 24337-1-PBS)

    TT cells were subjected to SDS PAGE followed by western blot with 24337-1-AP (CCDC176 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BBOF1 Polyclonal antibody (24337-1-AP)

    WB analysis using 24337-1-AP (same clone as 24337-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24337-1-AP (BBOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBOF1 Polyclonal antibody (24337-1-AP)

    IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using BBOF1 Polyclonal antibody (24337-1-AP)

    IHC staining of mouse testis using 24337-1-AP (same clone as 24337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24337-1-AP (BBOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24337-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

24337-1-PBS bindet in WB, IHC, Indirect ELISA BBOF1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BBOF1 fusion protein Ag19302
Vollständiger Name chromosome 14 open reading frame 45
Berechnetes Molekulargewicht 529 aa, 62 kDa
Beobachtetes Molekulargewicht62 kDa
GenBank-ZugangsnummerBC104981
Gene symbol BBOF1
Gene ID (NCBI) 80127
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Basal body orientation factor 1 (BBOF1), also known as CCDC176, contains two coiled-coil domains and is evolutionarily conserved in vertebrates. Recently, Xenopus bbof1 has been characterized as a cilia-associated factor that is induced by the transcription factor foxj1 to mediate the alignment and orientation of cilia in epidermal multi-ciliated cells. BBOF1 is required for sperm flagellar axoneme maintenance and male fertility in mice. BBOF1 expression is elevated during meiosis, and it is accumulated in round spermatids. BBOF1 deletion has compromised effects on growth, survival, and spermatogenesis.