BBS1 Polyklonaler Antikörper

BBS1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 21118-1-AP

Synonyme

Bardet Biedl syndrome 1, BBS1, BBS2 like protein 2, BBS2L2

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  WB analysis of mouse kidney using 21118-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21118-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 21118-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21118-1-AP (BBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21118-1-AP

  IP result of anti-BBS1 (IP:21118-1-AP, 4ug; Detection:21118-1-AP 1:600) with mouse brain tissue lysate 4000ug.

• 

  IF Staining of hTERT-RPE1 cells using 21118-1-AP

  IF result (base of ciliary stain) of anti-BBS1 (21118-1-AP, 1:50) with with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushirngewebe, Mausherzgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

21118-1-AP bindet in WB, IF/ICC, IP, ELISA BBS1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS1 fusion protein Ag15460
• Vollständiger Name: Bardet-Biedl syndrome 1
• Berechnetes Molekulargewicht: 593 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC047642
• Gene symbol: BBS1
• Gene ID (NCBI): 582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
human	IF	J Biol Chem; Non-redundant roles of DIAPHs in primary ciliogenesis.; Authors - Oliva Palander
		PLoS Biol; Melanocortin 1 receptor mediates melanin production by interacting with the BBSome in primary cilia; Authors - Xiaoyu Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jerome (Verified Customer) (05-07-2019)

Nuclear staining, and perinuclear staining that looks like a Golgi

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 100
• Cell Tissue Type: Hela Cells

BBS1 Antibody Immunofluorescence, validation (100 dilution) in Hela Cells (Cat no:21118-1-AP)