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BBS10 Polyklonaler Antikörper

BBS10 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12421-2-PBS

Synonyme

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  • Western Blot (WB) analysis of HepG2 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of HepG2 using 12421-2-AP (same clone as 12421-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of A549 using 12421-2-AP (same clone as 12421-2-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using BBS10 Polyclonal antibody (12421-2-AP)

    WB analysis of MCF-7 using 12421-2-AP (same clone as 12421-2-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using BBS10 Polyclonal antibody (12421-2-AP)

    IF Staining of hTERT-RPE1 cells using 12421-2-AP (same clone as 12421-2-PBS)

    IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12421-2-PBS bindet in WB, IF/ICC, Indirect ELISA BBS10 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BBS10 fusion protein Ag3073
Vollständiger Name Bardet-Biedl syndrome 10
Berechnetes Molekulargewicht 81 kDa
Beobachtetes Molekulargewicht 81 kDa
GenBank-ZugangsnummerBC013795
Gene symbol BBS10
Gene ID (NCBI) 79738
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).