BBS2 Polyklonaler Antikörper

BBS2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 11188-2-AP

Synonyme

Bardet Biedl syndrome 2, BBS, BBS2

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Galerie der Validierungsdaten

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  WB analysis using 11188-2-AP

  Mouse testis lysates were subjected to SDS PAGE followed by western blot with 11188-2-AP (BBS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11188-2-AP

  IP Result of anti-BBS2 (IP:11188-2-AP, 4ug; Detection:11188-2-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of hTERT-RPE1 cells using 11188-2-AP

  IF result (cilia stain) of anti-BBS2 (11188-2-AP, 1:50) with serum-starved hTERT-RPE1 cells by Dr. Moshe Kim.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Produktinformation

11188-2-AP bindet in WB, IP, IHC, IF, ELISA BBS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS2 fusion protein Ag1662
• Vollständiger Name: Bardet-Biedl syndrome 2
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC014140
• Gene symbol: BBS2
• Gene ID (NCBI): 583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS2 is one of seven BBS proteins that form the stable core of a protein complex (BBSome complex, 438kd) which is required for ciliogenesis but is dispensable for centriolar satellite function.

Publikationen

Species	Application	Title
human	IF	Cell; The conserved Bardet-Biedl syndrome proteins assemble a coat that traffics membrane proteins to cilia.; Authors - Jin Hua H
mouse	IF	Cell Stem Cell; Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.; Authors - Kusek Gretchen G
mouse	IF	J Cell Biol; Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.; Authors - Paurav B Desai
mouse	IHC	Brain Struct Funct; A novel form of ciliopathy underlies hyperphagia and obesity in Ankrd26 knockout mice.; Authors - Peter Acs
mouse	IF	Hum Mol Genet; Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse.; Authors - Jiang Si-Tse ST
mouse	IF	FASEB J; Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.; Authors - Wei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Deng (Verified Customer) (07-30-2019)

This antibody stained primary cilia in cultured IMCD3 cells.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1;250
• Cell Tissue Type: IMCD3 cells

BBS2 Antibody Immunofluorescence, validation (1;250 dilution) in IMCD3 cells (Cat no:11188-2-AP)