BBS4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BBS4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(21)

Rezensionen (1)

Kat-Nr. : 12766-1-AP

Synonyme

Bardet Biedl syndrome 4, Bardet-Biedl syndrome 4 protein

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  WB analysis using 12766-1-AP

  HEK-293 cell lysates were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12766-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12766-1-AP (BBS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 12766-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 12766-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12766-1-AP (BBS4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 cells using 12766-1-AP

  IF result (cytoplasm and the length of cilia cilia stain) of anti-BBS4 (12766-1-AP; 1:50) with serum-starved hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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  IF Staining of hTERT-RPE1 using 12766-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS4 antibody (12766-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maus-Magengewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 12 publications below

Produktinformation

12766-1-AP bindet in WB, IHC, IF/ICC, ELISA BBS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS4 fusion protein Ag3430
• Vollständiger Name: Bardet-Biedl syndrome 4
• Berechnetes Molekulargewicht: 519 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC027624
• Gene symbol: BBS4
• Gene ID (NCBI): 585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Coupling bimolecular PARylation biosensors with genetic screens to identify PARylation targets.; Authors - Dragomir B Krastev
mouse	WB,IF	PLoS Biol; Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons.; Authors - Naila Haq ; KO Validated
human	IF	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie
		J Cell Biol; SARS-CoV-2 ORF10 impairs cilia by enhancing CUL2ZYG11B activity.; Authors - Liying Wang
mouse	IF	Autophagy; The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.; Authors - Andreas Struchtrup
human	WB	EMBO Rep; Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.; Authors - Melis D Arslanhan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Detect 1 band above 47 kDa marker (expected size of BBS4 is 50-60 kDa), but also a much stronger band just below the 20 kDa marker

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HEK293T