BBS5 Monoklonaler Antikörper

BBS5 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G8E12

Kat-Nr. : 66136-1-PBS

Synonyme

Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein

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  WB analysis of human brain using 66136-1-Ig (same clone as 66136-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66136-1-Ig (BBS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66136-1-Ig (same clone as 66136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66136-1-Ig (same clone as 66136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66136-1-Ig (same clone as 66136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66136-1-Ig (same clone as 66136-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66136-1-Ig (BBS5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 66136-1-Ig (same clone as 66136-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using BBS5 antibody (66136-1-Ig, Clone: 4G8E12 ) at dilution of 1:3511 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66136-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66136-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA BBS5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BBS5 fusion protein Ag6844
• Vollständiger Name: Bardet-Biedl syndrome 5
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC044593
• Gene symbol: BBS5
• Gene ID (NCBI): 129880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.