BBS5 Rekombinanter Antikörper

BBS5 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240674B5

Kat-Nr. : 83722-1-RR

Synonyme

240674B5, Bardet Biedl syndrome 5, Bardet-Biedl syndrome 5 protein

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  WB analysis using 83722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83722-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83722-1-RR (BBS5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 83722-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using BBS5 antibody (83722-1-RR, Clone: 240674B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Retina-Gewebe, Maus-Augengewebe, Mausnierengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83722-1-RR bindet in WB, IF/ICC, ELISA BBS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BBS5 fusion protein Ag6153
• Vollständiger Name: Bardet-Biedl syndrome 5
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC044593
• Gene symbol: BBS5
• Gene ID (NCBI): 129880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS5 encodes a protein that has been directly linked to Bardet-Biedl syndrome. Bardet-Biedl syndrome (BBS) is an autosomal recessive condition characterised by rod-cone dystrophy, postaxial polydactyly, central obesity, mental retardation, hypogonadism, and renal dysfunction. Other associated clinical findings in BBS patients include diabetes, hypertension and congenital heart defects. BBS expression varies both within and between families and diagnosis is often difficult. Experimentation in non-human eukaryotes suggests that BBS5 is expressed in ciliated cells and that it is required for the formation of cilia. Alternate transcriptional splice variants have been observed but have not been fully characterized.