BBS7 Polyklonaler Antikörper

BBS7 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 18961-1-AP

Synonyme

Bardet Biedl syndrome 7, BBS2 like protein 1, BBS2L1, BBS7

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18961-1-AP (BBS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 18961-1-AP

  IP result of anti-BBS7 (IP:18961-1-AP, 4ug; Detection:18961-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18961-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18961-1-AP (BBS7 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

18961-1-AP bindet in WB, IHC, IF, IP, ELISA BBS7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS7 fusion protein Ag4601
• Vollständiger Name: Bardet-Biedl syndrome 7
• Berechnetes Molekulargewicht: 715 aa, 80 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC032691
• Gene symbol: BBS7
• Gene ID (NCBI): 55212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BBS7, also named as BBS2L1, is a component of the BBSome complex which is required for ciliogenesis but is dispensable for centriolar satellite function. BBS7 is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome.

Publikationen

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	PLoS Genet; A novel protein LZTFL1 regulates ciliary trafficking of the BBSome and Smoothened.; Authors - Seo Seongjin S
human	WB	PLoS Genet; The Centriolar Satellite Protein AZI1 Interacts with BBS4 and Regulates Ciliary Trafficking of the BBSome.; Authors - Xitiz Chamling
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
mouse	IHC, IP, WB	Hum Mol Genet; Photoreceptor cilia, in contrast to primary cilia, grant entry to a partially assembled BBSome.; Authors - Ying Hsu
mouse	IF	FASEB J; Rhodopsin mislocalization drives ciliary dysregulation in a novel autosomal dominant retinitis pigmentosa knock-in mouse model; Authors - Shimpei Takita