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BCAT1 Polyklonaler Antikörper

BCAT1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28622-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using BCAT1 Polyclonal antibody (28622-1-AP)

    WB analysis using 28622-1-AP (same clone as 28622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28622-1-AP (BCAT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human liver cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human lung cancer using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using BCAT1 Polyclonal antibody (28622-1-AP)

    IHC staining of human gliomas using 28622-1-AP (same clone as 28622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28622-1-AP (BCAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28622-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28622-1-PBS bindet in WB, IHC, Indirect ELISA BCAT1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BCAT1 fusion protein Ag28998
Vollständiger Name branched chain aminotransferase 1, cytosolic
Berechnetes Molekulargewicht 320 aa, 36 kDa
Beobachtetes Molekulargewicht 43-48 kDa
GenBank-ZugangsnummerBC033864
Gene symbol BCAT1
Gene ID (NCBI) 586
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).