BCAT1 Monoklonaler Antikörper

BCAT1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G3A10

Kat-Nr. : 67084-1-Ig

Synonyme

BCAT1, DKFZp686E12175, ECA39, MECA39, PNAS 121, PP18, Protein ECA39

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  WB analysis of Ramos using 67084-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67084-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 67084-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 67084-1-Ig (BCAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, Daudi-Zellen, Hausschwein-Hirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67084-1-Ig bindet in WB, ELISA BCAT1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCAT1 fusion protein Ag28328
• Vollständiger Name: branched chain aminotransferase 1, cytosolic
• Berechnetes Molekulargewicht: 320 aa, 36 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC033864
• Gene symbol: BCAT1
• Gene ID (NCBI): 586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).