BCAT2 Rekombinanter Antikörper

BCAT2 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251057E6

Kat-Nr. : 86367-1-PBS

Synonyme

Branched-chain-amino-acid aminotransferase, mitochondrial, EC:2.6.1.42, ECA40, Placental protein 18, PP18

---

• 

  WB analysis using 86367-1-RR (same clone as 86367-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86367-1-RR (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 86367-1-RR (same clone as 86367-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86367-1-RR (BCAT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86367-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86367-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86367-1-RR against Human BCAT2 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

86367-1-PBS bindet in WB, IHC, Indirect ELISA BCAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCAT2 fusion protein Ag9573
• Vollständiger Name: branched chain aminotransferase 2, mitochondrial
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC001900
• Gene symbol: BCAT2
• Gene ID (NCBI): 587
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).