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  • Featured Product
  • KD/KO Validated

BCCIP Polyklonaler Antikörper

BCCIP Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16043-1-PBS

Synonyme

TOK1, TOK 1, Protein TOK-1, Protein TOK 1, P21- and CDK-associated protein 1

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  • Western Blot (WB) analysis of HeLa cells using BCCIP Polyclonal antibody (16043-1-AP)

    WB analysis of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using BCCIP Polyclonal antibody (16043-1-AP)

    WB analysis of A549 using 16043-1-AP (same clone as 16043-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 16043-1-AP (BCCIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using BCCIP Polyclonal antibody (16043-1-AP)

    IP experiment of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

    IP result of anti-BCCIP (IP:16043-1-AP, 4ug; Detection:16043-1-AP 1:800) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCCIP Polyclonal antibody (16043-1-AP)

    IF Staining of HeLa using 16043-1-AP (same clone as 16043-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCCIP antibody (16043-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16043-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16043-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA BCCIP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BCCIP fusion protein Ag8934
Vollständiger Name BRCA2 and CDKN1A interacting protein
Berechnetes Molekulargewicht 314 aa, 36 kDa
Beobachtetes Molekulargewicht 50-58 kDa
GenBank-ZugangsnummerBC009771
Gene symbol BCCIP
Gene ID (NCBI) 56647
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCCIP (or TOK1) is isolated as a BRCA2- and CDKN1A (p21)-interacting protein and is implicated in homologous recombination (HR) pathway and inhibition of DNA replication stress. BCCIP is an evolutionarily conserved nuclear protein with multiple interacting domains. BCCIP deficiency in mice impaired embryonic and postnatal neural development, causing severe ataxia, cerebral and cerebellar defects, and microcephaly, which are associated with spontaneous DNA damage and subsequent cell death in the proliferative cell populations of the neural system during embryogenesis. BCCIP is essential for maintaining the transactivation activity of wild type p53 suggesting a potential role of BCCIP in cancer etiology.