BCDIN3D Rekombinanter Antikörper

BCDIN3D Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240152A9

Kat-Nr. : 83309-1-PBS

Synonyme

240152A9, BCDIN3 domain-containing protein, EC:2.1.1.-, RNA 5'-monophosphate methyltransferase

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  WB analysis using 83309-1-RR (same clone as 83309-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83309-1-RR (BCDIN3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83309-1-RR (same clone as 83309-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BCDIN3D Recombinant antibody (83309-1-RR, Clone:240152A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83309-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00333-1

  Cytometric bead array standard curve of MP00333-1, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-3-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00333-3

  Cytometric bead array standard curve of MP00333-3, BCDIN3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83309-1-PBS. Detection antibody: 83309-4-PBS. Standard: Ag15613. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83309-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83309-1-PBS against Human BCDIN3D was performed. The affinity constant is 3.04 nM.

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  Affinity and Kinetic Characterization of 83309-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83309-1-RR against Human BCDIN3D were performed. The affinity constant is 2.38 nM.

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Geprüfte Anwendungen

Produktinformation

83309-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA BCDIN3D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCDIN3D fusion protein Ag15613
• Vollständiger Name: BCDIN3 domain containing
• Berechnetes Molekulargewicht: 292 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC053560
• Gene symbol: BCDIN3D
• Gene ID (NCBI): 144233
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCDIN3D (BCDIN3 domain containing RNA methyltransferase), it is expected to be located in cytoplasm, which is ubiquitous expressed in prostate, lymph node and skeletal muscle. This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. The molecular weight of BCDIN3D is 33 kDa.