BCKDHA Polyklonaler Antikörper

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• KD/KO Validated

BCKDHA Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30028-1-PBS

Synonyme

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  WB analysis using 30028-1-AP (same clone as 30028-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30028-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 30028-1-AP (same clone as 30028-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 30028-1-AP (BCKDHA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30028-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 30028-1-AP (same clone as 30028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30028-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 30028-1-AP (same clone as 30028-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 30028-1-AP (BCKDHA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30028-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30028-1-PBS bindet in WB, IHC, Indirect ELISA BCKDHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCKDHA fusion protein Ag32613
• Vollständiger Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 42-50 kDa
• GenBank-Zugangsnummer: BC008933
• Gene symbol: BCKDHA
• Gene ID (NCBI): 593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).