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BCKDHA Rekombinanter Antikörper

BCKDHA Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250051G11

Kat-Nr. : 85799-3-PBS

Synonyme

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

Filter:
  • Western Blot (WB) analysis of various lysates using BCKDHA Recombinant antibody (85799-3-RR)

    WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using BCKDHA Recombinant antibody (85799-3-RR)

    WB analysis using 85799-3-RR (same clone as 85799-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85799-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85799-3-PBS bindet in WB, Indirect ELISA BCKDHA und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen BCKDHA fusion protein Ag32613
Vollständiger Name branched chain keto acid dehydrogenase E1, alpha polypeptide
Berechnetes Molekulargewicht 50 kDa
Beobachtetes Molekulargewicht42-50 kDa
GenBank-ZugangsnummerBC008933
Gene symbol BCKDHA
Gene ID (NCBI) 593
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).