BCKDHA Rekombinanter Antikörper

BCKDHA Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250051G11

Kat-Nr. : 85799-3-RR

Synonyme

2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, BCKDH E1-alpha, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, EC:1.2.4.4, MSU

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  WB analysis using 85799-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85799-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85799-3-RR (BCKDHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, HepG2-Zellen, Mausherzgewebe, Mauslebergewebe, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85799-3-RR bindet in WB, ELISA BCKDHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCKDHA fusion protein Ag32613
• Vollständiger Name: branched chain keto acid dehydrogenase E1, alpha polypeptide
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 42-50 kDa
• GenBank-Zugangsnummer: BC008933
• Gene symbol: BCKDHA
• Gene ID (NCBI): 593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), the gene encoding the regulated subunit of BCKDC was only one of two primary susceptibility genes identified that affected the risk of both type 2 diabetes mellitus (T2DM) and obesity (PMID: 25287287). BIX01294 transcriptionally downregulated the transcription of BCKDHA, which is essential for fueling the tricarboxylic acid (TCA) cycle. Studies have shown that KDM3A, a Jumonji histone demethylase, epigenetically regulates BCKDHA expression by binding to the BCKDHA gene promoter (PMID: 34876693). Moreover, at least four genes including BCKDHA, branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), dihydrolipoamide dehydrogenase (DLD), and dihydrolipoamide branched chain transacylase E2 (DBT) have been reported to be the causative gene for Maple syrup urine disease (MSUD) (PMID: 34187135).