BCKDK Polyklonaler Antikörper

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BCKDK Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15718-1-PBS

Synonyme

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, BCKDH kinase, BCKDHKIN, BDK, Branched-chain alpha-ketoacid dehydrogenase kinase

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  WB analysis of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  WB analysis using 15718-1-AP (same clone as 15718-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15718-1-AP (BCKDK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15718-1-AP (same clone as 15718-1-PBS)

  WB result of BCKDK antibody (15718-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCKDK transfected HEK-293 cells. This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 15718-1-AP (same clone as 15718-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15718-1-AP (BCKDK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15718-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15718-1-PBS bindet in WB, IHC, Indirect ELISA BCKDK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCKDK fusion protein Ag8395
• Vollständiger Name: branched chain ketoacid dehydrogenase kinase
• Berechnetes Molekulargewicht: 412 aa, 46 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC007363
• Gene symbol: BCKDK
• Gene ID (NCBI): 10295
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.