BCL10 Rekombinanter Antikörper

BCL10 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250149A6

Kat-Nr. : 85858-2-PBS

Synonyme

B-cell CLL/lymphoma 10, B-cell lymphoma/leukemia 10, Bcl 10, Bcl-10, c E10

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  WB analysis using 85858-2-RR (same clone as 85858-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85858-2-RR (BCL10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85858-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02160-2

  The mean BCL10 concentration was determined to be 105.67 ng/mL in Jurkat cell extract based on a 1.20 mg/mL extract load, 327.67 ng/mL in K-562 cell extract based on a 3.00 mg/mL extract load and 82.11 ng/mL in A549 cell extract based on a 1.10 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02160-2

  Sandwich ELISA standard curve of MP02160-2, Human BCL10 Recombinant Matched Antibody Pair - PBS only. 85858-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12170. 85858-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

• 

  Cytometric bead array standard curve of MP02160-2

  Cytometric bead array standard curve of MP02160-2, BCL10 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85858-3-PBS. Detection antibody: 85858-2-PBS. Standard: Ag12170. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85858-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85858-2-RR against Human BCL10 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85858-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA BCL10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCL10 fusion protein Ag12170
• Vollständiger Name: B-cell CLL/lymphoma 10
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC053617
• Gene symbol: BCL10
• Gene ID (NCBI): 8915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.