BCL11A Rekombinanter Antikörper

BCL11A Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242481F2

Kat-Nr. : 84930-3-PBS

Synonyme

242481F2, B cell lymphoma/leukaemia 11A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, BCL-11A

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  WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 84930-3-RR (same clone as 84930-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84930-3-RR (BCL11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01680-2

  Sandwich ELISA standard curve of MP01680-2, Human BCL11A Recombinant Matched Antibody Pair - PBS only. 84930-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2187. 84930-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01680-2

  The mean BCL11A concentration was determined to be 16.52 ng/mL in U-937 cell extract based on a 2.20 mg/mL extract load.

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  IF Staining of Jurkat using 84930-3-RR (same clone as 84930-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11A antibody (84930-3-RR, Clone: 242481F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84930-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01680-2

  Cytometric bead array standard curve of MP01680-2, BCL11A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84930-3-PBS. Detection antibody: 84930-4-PBS. Standard: Ag2187. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84930-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84930-3-RR against Human BCL11A were performed. The affinity constant is 1.38 nM.

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Geprüfte Anwendungen

Produktinformation

84930-3-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test BCL11A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCL11A fusion protein Ag2187
• Vollständiger Name: B-cell CLL/lymphoma 11A (zinc finger protein)
• Berechnetes Molekulargewicht: 835 aa, 91 kDa
• Beobachtetes Molekulargewicht: 110-120 kDa
• GenBank-Zugangsnummer: BC021098
• Gene symbol: BCL11A
• Gene ID (NCBI): 53335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.