BCL11B Polyklonaler Antikörper

BCL11B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 55414-1-AP

Synonyme

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

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• 

  WB analysis of Jurkat using 55414-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 55414-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 55414-1-AP

  IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug.

• 

  IHC staining of mouse brain using 55414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 55414-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen, Maus-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

55414-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BCL11B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: B-cell CLL/lymphoma 11B (zinc finger protein)
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: NM_022898
• Gene symbol: BCL11B
• Gene ID (NCBI): 64919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

Publikationen

Species	Application	Title
Mouse		Cell Rep; XLF/Cernunnos loss impairs mouse brain development by altering symmetric proliferative divisions of neural progenitors; Authors - Amandine Bery
human	WB	Arch Virol; Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.; Authors - Juan Wang
mouse	WB,IF	EMBO J; BMP suppresses Wnt signaling via the Bcl11b-regulated NuRD complex to maintain intestinal stem cells; Authors - Yehua Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

Good for ICC on subcerebral neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs