BCL11B Rekombinanter Antikörper

BCL11B Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242683B9

Kat-Nr. : 85363-1-RR

Synonyme

242683B9, B cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B-cell lymphoma/leukemia 11B

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  WB analysis using 85363-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85363-1-RR (BCL11B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 85363-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using BCL11B antibody (85363-1-RR, Clone: 242683B9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 85363-1-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug BCL11B Recombinant antibody (85363-1-RR, Clone:242683B9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MOLT-4-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85363-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA BCL11B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: B-cell CLL/lymphoma 11B (zinc finger protein)
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 116 kDa
• GenBank-Zugangsnummer: NM_022898
• Gene symbol: BCL11B
• Gene ID (NCBI): 64919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.