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  • Featured Product
  • KD/KO Validated

BCL2 Monoklonaler Antikörper

BCL2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

4H8C6

Kat-Nr. : CL594-60178

Synonyme

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated BCL2 Monoclonal antibody (CL594-60178)

    IF Staining of HeLa using CL594-60178

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60178 (BCL2 antibody) at dilution of 1:100.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®594-conjugated BCL2 Monoclonal antibody (CL594-60178)

    FC experiment of Jurkat using CL594-60178

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human BCL2 (CL594-60178, Clone:4H8C6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60178 bindet in IF, FC (Intra) BCL2 und zeigt Reaktivität mit Hausschwein, human

Getestete Reaktivität Hausschwein, human
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen BCL2 fusion protein Ag3508
Vollständiger Name B-cell CLL/lymphoma 2
Berechnetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC027258
Gene symbol BCL2
Gene ID (NCBI) 596
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 BCL2 antibody CL594-60178Protokoll herunterladen
FC protocol for CL594 BCL2 antibody CL594-60178Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen