BCL2 Rekombinanter Antikörper

BCL2 Rekombinant Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

3O5

Kat-Nr. : CL647-80313

Synonyme

BCL-2, 3O5

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  IF Staining of human appendicitis using CL647-80313

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 647 BCL2 antibody (CL647-80313, Clone: 3O5 ) at dilution of 1:200.

• 

  IF Staining of human appendicitis using CL647-80313

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 647 BCL2 antibody (CL647-80313, Clone: 3O5 ) at dilution of 1:200.

• 

  FC experiment of Jurkat using CL647-80313

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human BCL2 (CL647-80313, Clone:3O5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Appendizitis-Gewebe
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80313 bindet in IF-P, FC (Intra) BCL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCL2 fusion protein Ag3508
• Vollständiger Name: B-cell CLL/lymphoma 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC027258
• Gene symbol: BCL2
• Gene ID (NCBI): 596
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).