BCL2L14 Polyklonaler Antikörper

BCL2L14 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12846-1-PBS

Synonyme

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  WB analysis of A549 using 12846-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12846-1-AP (same clone as 12846-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12846-1-AP (BCL2L14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12846-1-AP (same clone as 12846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12846-1-AP (BCL2L14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 12846-1-AP (same clone as 12846-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12846-1-AP (BCL2L14 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 12846-1-AP (same clone as 12846-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug BCL2L14 Polyclonal antibody (12846-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12846-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12846-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA BCL2L14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL2L14 fusion protein Ag3926
• Vollständiger Name: BCL2-like 14 (apoptosis facilitator)
• Berechnetes Molekulargewicht: 327 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC025778
• Gene symbol: BCL2L14
• Gene ID (NCBI): 79370
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.