BCL3 Rekombinanter Antikörper

BCL3 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242233B11

Kat-Nr. : 84882-5-PBS

Synonyme

242233B11, B-cell lymphoma 3 protein, BCL-3, BCL4, D19S37

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  IHC staining of mouse spleen using 84882-5-RR (same clone as 84882-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84882-5-RR (same clone as 84882-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84882-5-RR (BCL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84882-5-RR (same clone as 84882-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84882-5-RR (same clone as 84882-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BCL3 antibody (84882-5-RR, Clone: 242233B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84882-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84882-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84882-5-RR against Human BCL3 were performed. The affinity constant is 0.581 nM.

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Geprüfte Anwendungen

Produktinformation

84882-5-PBS bindet in IHC, IF/ICC, Indirect ELISA BCL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCL3 fusion protein Ag13375
• Vollständiger Name: B-cell CLL/lymphoma 3
• Berechnetes Molekulargewicht: 446 aa, 47 kDa
• Beobachtetes Molekulargewicht: 48-60 kDa
• GenBank-Zugangsnummer: BC064993
• Gene symbol: BCL3
• Gene ID (NCBI): 602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain (PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248).