BCL6 Monoklonaler Antikörper

BCL6 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6B1

Kat-Nr. : 66340-1-PBS

Synonyme

BCL-6, 1E6B1, B cell CLL/lymphoma 6, B cell lymphoma 6 protein, B-cell lymphoma 5 protein

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  WB analysis of Ramos using 66340-1-Ig (same clone as 66340-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 66340-1-Ig (same clone as 66340-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66340-1-Ig (same clone as 66340-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66340-1-Ig (BCL6 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66340-1-Ig (same clone as 66340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66340-1-Ig (BCL6 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66340-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66340-1-PBS bindet in WB, IHC, Indirect ELISA BCL6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BCL6 fusion protein Ag15519
• Vollständiger Name: B-cell CLL/lymphoma 6
• Berechnetes Molekulargewicht: 706 aa, 79 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC150184
• Gene symbol: BCL6
• Gene ID (NCBI): 604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.