BCL7B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCL7B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11740-1-AP

Synonyme

B cell CLL/lymphoma 7B, BCL7B, Hom s 3

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11740-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11740-1-AP

  WB result of BCL7B antibody (11740-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL7B transfected HeLa cells.

• 

  WB analysis of human brain using 11740-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11740-1-AP (BCL7B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11740-1-AP (BCL7B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11740-1-AP (BCL7B antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11740-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11740-1-AP (BCL7B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

11740-1-AP bindet in WB, IHC, IF, CoIP, ELISA BCL7B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL7B fusion protein Ag2333
• Vollständiger Name: B-cell CLL/lymphoma 7B
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC000956
• Gene symbol: BCL7B
• Gene ID (NCBI): 9275
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Olaquindox-Induced Liver Damage Involved the Crosstalk of Oxidative Stress and p53 In Vivo and In Vitro.; Authors - Daowen Li
mouse	WB	Aging (Albany NY); S-allyl cysteine reduces osteoarthritis pathology in the tert-butyl hydroperoxide-treated chondrocytes and the destabilization of the medial meniscus model mice via the Nrf2 signaling pathway.; Authors - Zhenxuan Shao
mouse	WB,IHC	Sci Rep; The SWI/SNF subunit Bcl7a contributes to motor coordination and Purkinje cell function.; Authors - Lena Wischhof ; KO Validated
human	WB,CoIP	Life Sci Alliance; BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression; Authors - Nicholas Dietrich ; KD Validated