BCL9 Polyklonaler Antikörper

BCL9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 22947-1-AP

Synonyme

Bcl-9, Protein legless homolog, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein

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  WB analysis of Jurkat using 22947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22947-1-AP (BCL9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 22947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22947-1-AP (BCL9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22947-1-AP (BCL9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22947-1-AP (BCL9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (22947-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

22947-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BCL9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCL9 fusion protein Ag19158
• Vollständiger Name: B-cell CLL/lymphoma 9
• Berechnetes Molekulargewicht: 1426 aa, 149 kDa
• Beobachtetes Molekulargewicht: 149 kDa
• GenBank-Zugangsnummer: BC116451
• Gene symbol: BCL9
• Gene ID (NCBI): 607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Publikationen

Species	Application	Title
human	WB	Bioorg Chem; Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway; Authors - Yongxi Dong
human,mouse	WB,IHC	Reprod Biol Endocrinol; Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.; Authors - Xi Wang
human	WB,IF,IP	J Biol Chem; USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.; Authors - Zesen Shang