BCLAF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

BCLAF1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 26809-1-AP

Synonyme

KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1

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  WB analysis of Jurkat using 26809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 26809-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 26809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 26809-1-AP (BCLAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 26809-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26809-1-AP (BCLAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 26809-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26809-1-AP (BCLAF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26809-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26809-1-AP (BCLAF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26809-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26809-1-AP (BCLAF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

26809-1-AP bindet in WB, IHC, IF, RIP, ELISA BCLAF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCLAF1 fusion protein Ag25210
• Vollständiger Name: BCL2-associated transcription factor 1
• Berechnetes Molekulargewicht: 920 aa, 106 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC132780
• Gene symbol: BCLAF1
• Gene ID (NCBI): 9774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

Publikationen

Species	Application	Title
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
human	WB	Aging (Albany NY); LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA.; Authors - Mingwei Chen
human	WB,IHC,IF	Transl Oncol; Hsp90 Inhibitor STA9090 induced VPS35 related extracellular vesicle release and metastasis in hepatocellular carcinoma; Authors - Wenchong Tan ; KD Validated
mouse	WB	eLife; SRSF10 is essential for progenitor spermatogonia expansion by regulating alternative splicing; Authors - Wenbo Liu
human	WB	J Oncol; The Comprehensive Analysis of N6-Methyadenosine Writer METTL3 and METTL14 in Gastric Cancer; Authors - Yiling Ge
human	WB	Biochem Genet; BCLAF1 is Expressed as a Potential Anti-oncogene in Bile Duct Cancer; Authors - Yutong Zhou