BCOR Polyklonaler Antikörper

BCOR Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 12107-1-AP

Synonyme

ANOP2, BCOR, KIAA1575, MAA2, MCOPS2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, K-562-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12107-1-AP bindet in WB, IHC, IP, CoIP, ChIP, ELISA BCOR und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BCOR fusion protein Ag2752
Vollständiger Name BCL6 co-repressor
Berechnetes Molekulargewicht 1703 aa, 186 kDa
Beobachtetes Molekulargewicht 186 kDa
GenBank-ZugangsnummerBC009675
Gene symbol BCOR
Gene ID (NCBI) 54880
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCOR, a corepressor of BCL6, binds to transcriptional factors and specifically inhibits gene expression. BCOR plays a key role in the early embryonic development, mesenchymal stem cell function and hematopiesis. BCOR can associate with HDACs, the polycomb group protein, polycomb group Ring finger protein1/nervous system polycomb1 (PCGF1/NSPC1), histone demethylase, F-box- and leucine-rich repeat protein10(FBXL10). Via repression of TFAP2A acts as a negative regulator of osteo-dentiogenic capacity in adult stem

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for BCOR antibody 12107-1-APProtokoll herunterladen
IHC protocol for BCOR antibody 12107-1-APProtokoll herunterladenl
IP protocol for BCOR antibody 12107-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB, IHC

Nat Genet

Consistent in-frame internal tandem duplications of BCOR characterize clear cell sarcoma of the kidney.

Authors - Hitomi Ueno-Yokohata
humanWB

Blood

Epigenetic Regulation of Non-canonical Menin Targets Modulates Menin Inhibitor Response in Acute Myeloid Leukemia

Authors - Xinyue Zhou
human,mouse

Mol Cell

Non-canonical PRC1.1 licenses transcriptional response to enable Treg plasticity in immune adaptation

Authors - Ting Li
humanWB

Nucleic Acids Res

Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis.

Authors - Dongxue Su
mouseWB

Int J Mol Sci

BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.

Authors - Satoshi Nakata
mouseWB

Sci Rep

Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells.

Authors - Yun Yan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Sid (Verified Customer) (05-13-2019)

Works well for mouse hematopoetic cells, some strong non specific bands ~ 150 kD and ~100 kD, however can be reasonably resolved on 4-15% gel.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse hematopoetic