BCORL1 Polyklonaler Antikörper

BCORL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24315-1-AP

Synonyme

CXorf10

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  WB analysis using 24315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 24315-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HuH-7-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24315-1-AP bindet in WB, IHC, IF/ICC, ELISA BCORL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BCORL1 fusion protein Ag19522
• Vollständiger Name: BCL6 co-repressor-like 1
• Berechnetes Molekulargewicht: 1711 aa, 183 kDa
• Beobachtetes Molekulargewicht: 200 kDa
• GenBank-Zugangsnummer: BC156742
• Gene symbol: BCORL1
• Gene ID (NCBI): 63035
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.