BCRP/ABCG2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

BCRP/ABCG2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240959A4

Kat-Nr. : 83835-1-RR

Synonyme

ABCG2, BCRP, BCRP,ABCG2, 240959A4, ABC15

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  WB analysis using 83835-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83835-1-RR (BCRP,ABCG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83835-1-RR

  WB result of BCRP/ABCG2 antibody (83835-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCRP/ABCG2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83835-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83835-1-RR against Human BCRP,ABCG2 were performed. The affinity constant is 9.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, Maushirngewebe, SGC-7901-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83835-1-RR bindet in WB, ELISA BCRP/ABCG2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BCRP/ABCG2 fusion protein Ag25911
• Vollständiger Name: ATP-binding cassette, sub-family G (WHITE), member 2
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 68-72 kDa
• GenBank-Zugangsnummer: BC021281
• Gene symbol: ABCG2
• Gene ID (NCBI): 9429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BCRP (breast cancer resistance protein) confers a broad spectrum of drug resistance and the expression of BCRP is enhanced in several cancer cell models. It has ATPase activity and belongs to a family of multiple drug resistant proteins. Data also indicated that BCRP might be an early marker for some stem cells.