BDH2 Polyklonaler Antikörper

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BDH2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 27207-1-AP

Synonyme

(R)-beta-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6, EC:1.1.1.-

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  WB analysis of HeLa using 27207-1-AP

  WB result of BDH2 antibody (27207-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

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  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27207-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

27207-1-AP bindet in WB, IHC, ELISA BDH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BDH2 fusion protein Ag24934
• Vollständiger Name: 3-hydroxybutyrate dehydrogenase, type 2
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC095414
• Gene symbol: BDH2
• Gene ID (NCBI): 56898
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Type 2-hydroxybutyrate dehydrogenase (BDH2) is a short-chain dehydrogenase/reductase family member involved in several biological and pathological processes, including the utilization of cytosolic ketone bodies, immunocyte regulation and tumor progression. As a member of a gene family involved in ketone metabolism, BDH2 upregulated the level of β-HB in liver cells as well as the level of H3 histone acetylation (PMID: 25762501). Downregulation of BDH2 during infection is due to activation of hepcidin and intracellular iron retention, indicating an important role for BDH2 in iron-limiting innate immunity and host defense (PMID: 31333791, 32344823).

Publikationen

Species	Application	Title
human	WB	J Clin Invest; Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.; Authors - Xiaofei Gao
mouse,human	WB,IHC	J Hematol Oncol; Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.; Authors - Tianxiao Yang ; KD Validated
human	IHC	J Exp Clin Cancer Res; BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.; Authors - Jia-Zhou Liu
mouse	WB	J Proteome Res; 3-Hydroxybutyrate Promotes Myoblast Proliferation and Differentiation through Energy Metabolism and GPR109a-Mediated Ca2+-NFAT Signaling Pathways; Authors - Xu Qiu
human	WB	Nat Metab; BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states; Authors - Francesca Rizzollo ; KD Validated