BDNF Polyklonaler Antikörper

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• KD/KO Validated

BDNF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25699-1-PBS

Synonyme

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form

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  WB analysis using 25699-1-AP (same clone as 25699-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25699-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 25699-1-AP (same clone as 25699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25699-1-AP (same clone as 25699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 25699-1-AP (same clone as 25699-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25699-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 25699-1-AP (same clone as 25699-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (25699-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25699-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25699-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA BDNF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BDNF fusion protein Ag22489
• Vollständiger Name: brain-derived neurotrophic factor
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC029795
• Gene symbol: BDNF
• Gene ID (NCBI): 627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).