BDNF Polyklonaler Antikörper

BDNF Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(155)

Rezensionen (2)

Kat-Nr. : 28205-1-AP

Synonyme

BDNF precursor form, brain derived neurotrophic factor, Brain-derived neurotrophic factor

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  WB analysis using 28205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 28205-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C6-Zellen, Maus-Cerebellum-Gewebe, PC-12-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 139 publications below
• IHC: See 10 publications below
• IF: See 22 publications below

Produktinformation

28205-1-AP bindet in WB, IHC, IF, ELISA BDNF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BDNF fusion protein Ag28276
• Vollständiger Name: brain-derived neurotrophic factor
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC029795
• Gene symbol: BDNF
• Gene ID (NCBI): 627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publikationen

Species	Application	Title
		Autophagy; Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.; Authors - Paola Rusmini
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
mouse	WB	ACS Appl Mater Interfaces; Reactive Oxygen Species Scavenging Functional Hydrogel Delivers Procyanidins for the Treatment of Traumatic Brain Injury in Mice.; Authors - Xuyang Huang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB,IHC,IF	Oncogene; TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD; Authors - Linqing Sun
rat	WB	Phytother Res; Neuroinflammation inhibition and neuroprotective effects of purpurin, a potential anti-AD compound, screened via network proximity and gene enrichment analyses; Authors - Jun Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marine (Verified Customer) (07-01-2025)

This Ac worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1/500 and 1/1000
• Cell Tissue Type: Tumoral cells, endothelial cells, mouse fibroblasts

FH

Tanusree (Verified Customer) (12-18-2019)

Worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain