BDNF Monoklonaler Antikörper

BDNF Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(47)

CloneNo.

1E5C5

Kat-Nr. : 66292-1-Ig

Synonyme

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

---

• 

  WB analysis of U-251 using 66292-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66292-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 66292-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, fetales humanes Hirngewebe
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 37 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• FC: See 1 publications below

Produktinformation

66292-1-Ig bindet in WB, IHC, IF/ICC, ELISA BDNF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Zebrafisch
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BDNF fusion protein Ag11329
• Vollständiger Name: brain-derived neurotrophic factor
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC029795
• Gene symbol: BDNF
• Gene ID (NCBI): 627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publikationen

Species	Application	Title
human	IHC	Cancer Lett; Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.; Authors - Yansong Liu
		Oxid Med Cell Longev; l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways.; Authors - Li Zeng
	IF	Int J Mol Sci; Methotrexate Neurotoxicity Is Related to Epigenetic Modification of the Myelination Process.; Authors - Yu-Chieh Chen
	WB	ACS Chem Neurosci; Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus; Authors - Yan Sun
zebrafish	WB	Chemosphere; Perfluorooctane sulfonates induces neurobehavioral changes and increases dopamine neurotransmitter levels in zebrafish larvae.; Authors - Luyin Wu
	WB	Int Immunopharmacol; Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model; Authors - Xin-Mei Xie