Osteocalcin/OCN Polyklonaler Antikörper

Osteocalcin/OCN Polyklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 16157-1-AP

Synonyme

BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 16157-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin/OCN antibody (16157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 16157-1-AP

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody (16157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Osteosarkomgewebe, Mauslungengewebe, Rattenlungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Produktinformation

16157-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Osteocalcin/OCN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: bone gamma-carboxyglutamate (gla) protein
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_199173
• Gene symbol: Osteocalcin
• Gene ID (NCBI): 632
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	IF	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu
mouse	IF	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
mouse	IHC	Front Pharmacol; Naringenin is a Potential Anabolic Treatment for Bone Loss by Modulating Osteogenesis, Osteoclastogenesis, and Macrophage Polarization.; Authors - Xin Zhou
mouse	IHC	Bone; Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway; Authors - Ping Li
mouse	WB	J Cell Mol Med; KIAA0753 enhances osteoblast differentiation suppressed by diabetes; Authors - Mengxue Li
rat	IF	Sci Rep; Ginsenoside Rg1 regulating inflammatory response and bone-remodeling through Keap1/Nrf2 signaling pathway in rats with periodontitis; Authors - Yang Zhou