BHMT Rekombinanter Antikörper

BHMT Rekombinant Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250978E3

Kat-Nr. : 86372-1-PBS

Synonyme

Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5

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  WB analysis using 86372-1-RR (same clone as 86372-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86372-1-RR (BHMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

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  IP experiment of rat liver using 86372-1-RR (same clone as 86372-1-PBS)

  IP result of anti-BHMT (IP:86372-1-RR, 4ug; Detection:86372-1-RR 1:3000) with rat liver tissue lysate 3080 ug. This data was developed using the same antibody clone with 86372-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86372-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86372-1-RR against Human BHMT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86372-1-PBS bindet in WB, IP, Indirect ELISA BHMT und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BHMT fusion protein Ag8738
• Vollständiger Name: betaine-homocysteine methyltransferase
• Berechnetes Molekulargewicht: 406 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC012616
• Gene symbol: BHMT
• Gene ID (NCBI): 635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that Involved in the regulation of homocysteine metabolism. BHMT catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline(PMID: 8798461, 10529246).