BID Monoklonaler Antikörper

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BID Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A2E5

Kat-Nr. : 60301-1-PBS

Synonyme

p22 BID, 2A2E5, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11

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  WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

  WB result of BID antibody (60301-1-Ig, 1:8000) with si-Control and si-BID transfected HeLa cells. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60301-1-Ig (same clone as 60301-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60301-1-Ig (BID Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60301-1-Ig (same clone as 60301-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60301-1-Ig (BID Antibody) at dilution of 1:900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60301-1-Ig (same clone as 60301-1-PBS)

  Immunofluorescent analysis of HeLa cells using 60301-1-Ig (BID antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60301-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60301-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA BID und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: BID fusion protein Ag21005
• Vollständiger Name: BH3 interacting domain death agonist
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC009197
• Gene symbol: BID
• Gene ID (NCBI): 637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606).