BIN2 Polyklonaler Antikörper

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BIN2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14245-1-PBS

Synonyme

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  WB analysis using 14245-1-AP (same clone as 14245-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 14245-1-AP (same clone as 14245-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14245-1-AP (BIN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 14245-1-AP (same clone as 14245-1-PBS)

  IP result of anti-BIN2 (IP:14245-1-AP, 4ug; Detection:14245-1-AP 1:400) with Jurkat cells lysate 2000ug. This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 14245-1-AP (BIN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 14245-1-AP (same clone as 14245-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 14245-1-AP (BIN2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14245-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14245-1-PBS bindet in WB, IHC, IP, Indirect ELISA BIN2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BIN2 fusion protein Ag5502
• Vollständiger Name: bridging integrator 2
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC047686
• Gene symbol: BIN2
• Gene ID (NCBI): 51411
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAR proteins, which are characterized by a common N-terminal BAR domain, are a family of adaptor proteins implicated in a diverse set of cellular processes. BIN1 (bridging integrator 1) and BIN2 are BAR proteins. The human BIN2 gene is located at chromosome 4q22.1, and encodes a 565-amino acid protein with a predicted molecular weight of ~62 kDa. It shows preferential expression in hematopoietic tissues, with highest mRNA levels in spleen and peripheral blood leukocytes and also high levels in thymus, colon, and placenta. BIN2 has been shown to migrate with an apparent mobility of ~80 kDa, and interact with BIN1. (PMID: 10903846)