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BIN3 Polyklonaler Antikörper

BIN3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11215-1-PBS

Synonyme

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  • Western Blot (WB) analysis of K-562 cells using BIN3 Polyclonal antibody (11215-1-AP)

    WB analysis of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using BIN3 Polyclonal antibody (11215-1-AP)

    WB analysis of HeLa using 11215-1-AP (same clone as 11215-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using BIN3 Polyclonal antibody (11215-1-AP)

    IP experiment of K-562 using 11215-1-AP (same clone as 11215-1-PBS)

    IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using BIN3 Polyclonal antibody (11215-1-AP)

    IHC staining of human prostate cancer using 11215-1-AP (same clone as 11215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11215-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11215-1-PBS bindet in WB, IHC, IP, Indirect ELISA BIN3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BIN3 fusion protein Ag1713
Vollständiger Name bridging integrator 3
Berechnetes Molekulargewicht 30 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC009824
Gene symbol BIN3
Gene ID (NCBI) 55909
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).