BLIMP1/PRDM1 Rekombinanter Antikörper

BLIMP1/PRDM1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242030B1

Kat-Nr. : 84625-3-RR

Synonyme

BLIMP1, PRDM1, 242030B1, BLIMP 1, PRDI BF1

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  WB analysis using 84625-3-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 84625-3-RR (BLIMP1/PRDM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 84625-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 84625-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using BLIMP1/PRDM1 antibody (84625-3-RR, Clone: 242030B1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84625-3-RR bindet in WB, IF/ICC, ELISA BLIMP1/PRDM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: BLIMP1/PRDM1 fusion protein Eg2625
• Vollständiger Name: PR domain containing 1, with ZNF domain
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 96-110 kDa
• GenBank-Zugangsnummer: NM_001198.3
• Gene symbol: PRDM1
• Gene ID (NCBI): 639
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLIMP1(B lymphocyte-induced maturation protein 1), also known as PRDM1(PR domain zinc finger protein 1), is an evolutionarily conserved transcriptional regulator originally described as a repressor of gene transcription(PMID: 35154079). BLIMP1/PRDM1 is widely expressed in multiple cell types including B lymphocytes, T lymphocytes, mammary luminal progenitor cells and skin epithelial cells. BLIMP1/PRDM1 is a master regulator of terminal B cell differentiation and may also function as a tumor suppressor in the pathogenesis of DLBCL, where it is frequently inactivated by mutations and deletions.