BLK Polyklonaler Antikörper

BLK Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10510-1-PBS

Synonyme

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  WB analysis of Jurkat using 10510-1-AP (same clone as 10510-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10510-1-AP (same clone as 10510-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10510-1-AP (BLK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  IP experiment of SH-SY5Y using 10510-1-AP (same clone as 10510-1-PBS)

  IP result of anti-BLK (IP:10510-1-AP, 4ug; Detection:10510-1-AP 1:500) with SH-SY5Y cells lysate 1600ug. This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 10510-1-AP (same clone as 10510-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10510-1-AP (BLK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10510-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10510-1-PBS bindet in WB, IHC, IP, Indirect ELISA BLK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLK fusion protein Ag0795
• Vollständiger Name: B lymphoid tyrosine kinase
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC007371
• Gene symbol: BLK
• Gene ID (NCBI): 640
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosine-protein kinase Blk(BLK) is typically involved in cell proliferation and differentiation.BLK is a 58 kDa protein with important role in B-cell receptor signaling and B-cell development. BLK also stimulates INS synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.

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