BLMH Polyklonaler Antikörper

BLMH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14941-1-PBS

Synonyme

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  WB analysis of human colon using 14941-1-AP (same clone as 14941-1-PBS)

  human colon tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 14941-1-AP (same clone as 14941-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 14941-1-AP (same clone as 14941-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14941-1-AP (BLMH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse skin using 14941-1-AP (same clone as 14941-1-PBS)

  IP result of anti-BLMH (IP:14941-1-AP, 4ug; Detection:14941-1-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 14941-1-AP (same clone as 14941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14941-1-AP (BLMH antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14941-1-AP (same clone as 14941-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14941-1-AP (BLMH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14941-1-AP (same clone as 14941-1-PBS)

  Immunofluorescent analysis of Hela cells, using BLMH antibody 14941-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14941-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14941-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA BLMH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BLMH fusion protein Ag6888
• Vollständiger Name: bleomycin hydrolase
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC003616
• Gene symbol: BLMH
• Gene ID (NCBI): 642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLMH(Bleomycin hydrolase) is also named as BH, BMH and belongs to the peptidase C1 family. The gene is expressed in many human tissues with elevated expression levels found in testis, skeletal muscle, and pancreas, and low levels of expression in colon and peripheral blood leukocytes(PMID:8639621). The protein catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. BH-immunoreactivity is essentially located in the cytoplasm and at the outer membrane of the rough endoplasmic reticulum of epithelial cells of the kidney, as well as in that of hepatocytes of the liver(PMID:17158865).